|
KMID : 1098420130210020136
|
|
Korean Journal of Medicinal Crop Science
2013 Volume.21 No. 2 p.136 ~ p.141
|
|
|
Antioxidant Activity and Its Mechanism of Chelidonium majus Extract
|
|
Heo Jee-In
Kim Jeong-Hyeon
Lee Jeong-Min
Lim Soon-Sung
Kim Sung-Chan
Park Jae-Bong
Kim Jae-Bong
Lee Jae-Yong
|
|
|
Abstract
|
|
|
|
Chelidonium majus (CM) contains several isoquinoline alkaloids that have been reported to have various iological activities such as anti-inflammatory, antimicrobial, antioxidant, immune-modulatory, and antitumoral. It has been reported that the extract of CM had an antioxidant potential, however the mechanism has not been verified. In this study, we found that CM extract activated FOXO3a. FOXO3a is a transcription factor that involved in various biological processes such as cell cycle arrest, apoptosis, DNA repair, and ROS detoxification. Transcriptional activities of FOXO3a were regulated by post-translational modifications including phosphorylation, acetylation, and ubiquitination. Protein level of FOXO3a was increased by CM extract. Promoter activities of FOXO-transcriptional target genes such as MnSOD, p27 and GADD45 were activated by CM extract in a dose dependent manner. In addition, protein level of MnSOD, major antioxidant enzyme, was increased by CM extract. Thereby ROS level was decreased by CM in old HEF cells. These results suggest that CM extract has an antioxidant activity through FOXO activation.
|
|
|
KEYWORD
|
|
|
Chelidonium majus, FOXO3a, Antioxidant
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
|
|